Lundi 19 mai 2025 / Monday May, 19th 2025
|
| 15:30 - 16:00 |
Reception of participants |
| 16:00 - 16:15 |
Welcome and presentation by the organizers |
| SESSION I |
SAMPLE PREPARATION AND DATA ACQUISITION FOR CRYO ELECTRON TOMOGRAPHY |
| 16:15 - 17:00 |
Preparation of cell and tissue specimen for cryo-electron tomography |
| Bettina Zens (ISTA, Klosterneuburg, Austria) |
| 17:00 - 17:30 |
Coffee break |
| 17:30 - 18:15 |
Microscope setup and tomogram acquisition strategies |
| Wim Hagen (Thermo Fisher Scientific, Einhoven, Netherlands) |
| 18:15 - 19:00 |
Automated Data Acquisition with SerialEM: Tilt Series and Beyond |
| Günter Resch (Nexperion, Vienna, Austria) |
| 19:30 |
Dinner |
Mardi 20 mai 2025 / Tuesday May, 20th 2025
|
| 06:30 - 08:30 |
Breakfast |
| SESSION II |
CORRELATING LIGHT MICROSCOPY AND CRYO-ELECTRON TOMOGRAPHY |
| 08:30 - 09:15 |
Correlative Light, Electron, soft X-ray imaging techniques (CLEXM): principles and application on a model system |
| Pierre Montaville (Synchrotron Soleil, Gif Sur Yvette, France) |
| 09:15 - 10:00 |
CLEM and super resolution cryo fluorescence microscopy |
| Rainer Kaufmann (CSSB, Hamburg, Germany) |
| 10:00 - 10:30 |
Coffee break |
| SESSION III |
DATA PRE-PROCESSING, TOMOGRAM RECONSTRUCTION |
| 10:30 - 11:15 |
Preprocessing and tomogram reconstruction |
| Beata Turoňová (MPI of Biophysics, Frankfurt, Germany) |
| 11:15 - 12:00 |
Filtering, denoising and other approaches for improved tomogram visualization |
| Florian Fäßler (IGBMC, Strasbourg, France) |
| 12:00 - 14:00 |
Lunch |
| SESSION IV |
ANALYSIS STRATEGIES FOR CRYO-ELECTRON TOMOGRAPHY DATA SETS |
| 14:00 - 14:45 |
Overview of analysis possibilities, application and targeting strategies |
| Lindsay Baker (Kavli Institute for Nanoscience Discovery, Oxford, UK) |
| 14:45 - 15:30 |
Multiscale analysis through segmentation and subvolume averaging |
| Vojtěch Prazák (CSSB, Hamburg, Germany) |
| 15:30 - 16:00 |
Coffee Break |
| 16:00 - 16:45 |
TBD |
| Daniel Castaño Díez (INSTITUTO BIOFISIKA CSIC, Leioa, Spain) |
| 16:45 - 17:30 |
Cryo-ET at full Warp 2 |
| Dimitry Tegunov (Genentech, San Francisco, USA) |
| 17:30 - 18:15 |
High-resolution structure determination in situ |
| Florian Schur (ISTA, Klosterneuburg, Austria) |
| 19:30 - 20:15 |
Cocktail |
| 20:15 |
Dinner |
Mercredi 21 mai 2025 / Wednesday May, 21st 2025
|
| 06:30 - 08:30 |
Breakfast |
| SESSION V |
RECENT RESEARCH HIGHLIGHTS AND INTEGRATIVE APPROACHES |
| 08:30 - 09:15 |
In situ cryo-electron tomography of influenza A virus replication and assembly |
| Petr Chlanda (BioQuant, Heidelberg, Germany) |
| 09:15 - 10:00 |
The multi-scale architecture of mammalian sperm tail as revealed by cryo-electron tomography and single particle analysis |
| Tzviya Zeev-Ben-Mordehai (Utrecht University, Utrecht, Netherlands) |
| 10:00 - 10:30 |
Coffee Break |
| 10:30 - 11:15 |
CryoET insights into mRNA translation and related processes |
| Juliette Fedry (MRC LMB, Cambridge, UK) |
| 11:15 - 12:00 |
Biomolecular conformational landscapes by cryo-ET data analysis based on MD simulations |
| Slavica Jonic (IMPMC, Paris, France) |
| 12:00 - 14:00 |
Lunch |
| 14:00 |
Departure |
